Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)
Specificity
The antibody reacts with Linker for activation of T cells (LAT), a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. Does not react with mouse.
Characteristics
Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1
Purification
Protein A affinity chromatography
Purity
> 95 % (by SDS-PAGE)
Immunogen
Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmicdomain of human LAT
LAT
Reactivity: Human, Mouse, Rat
WB, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunoprecipitation. Western Blot (Tyrosine-phosphorylated form of LAT is not optimally recognized). Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
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Background
LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FceRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36