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Blood Group Lewis B antibody

Reactivity: Human IHC (p), WB, EIA Host: Mouse Monoclonal 2-25LE unconjugated
Catalog No. ABIN120686
  • Target See all Blood Group Lewis B products
    Blood Group Lewis B
    Reactivity
    • 13
    • 11
    • 9
    Human
    Host
    • 13
    Mouse
    Clonality
    • 13
    Monoclonal
    Conjugate
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Blood Group Lewis B antibody is un-conjugated
    Application
    • 12
    • 9
    • 9
    • 4
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography on Protein A.
    Immunogen
    Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)
    Clone
    2-25LE
    Isotype
    IgG1
  • Application Notes
    Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH 7.4, with 15 mM Sodium Azide as preservative.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2-8 °C for 6 months. For long-term storage aliquot and store at -20 °C. Avoid freeze/thaw cycles.
    Shelf Life: one year from despatch.
    Expiry Date
    12 months
  • Target
    Blood Group Lewis B
    Abstract
    Blood Group Lewis B Products
    Synonyms
    MUC5 antibody, TBM antibody, leB antibody, mucin 5AC, oligomeric mucus/gel-forming antibody, MUC5AC antibody
    Background
    Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Synonyms: Blood Group Antigen Lewis b
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