CD63 antibody

Catalog No. ABIN120614

Product Details anti-CD63 Antibody

Target: CD63

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)

Specificity: The antibody reacts with CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: HPB-ALL T cell line

Clone: MEM-259

Isotype: IgG1

Application Details

Application Notes: Flow cytometry (detection of activated platelets, neutrophils and basophils). Immunoprecipitation. Western blotting under non-reducing conditions. Immunocytochemistry. Immunohistochemistry (paraffin sections): Recommended dilution:10 m g/mL, Positive tissue: spleen
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. General.

Expiry Date: 12 months

References for anti-CD63 antibody (ABIN120614)

Schmidt, Gelhaus, Nebendahl, Lettau, Lucius, Leippe, Kabelitz, Janssen: "Effector granules in human T lymphocytes: the luminal proteome of secretory lysosomes from human T cells." in: Cell communication and signaling : CCS, Vol. 9, Issue 1, pp. 4, (2011) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63 (CD63 Products)

Synonyms: C75951 antibody, ME491 antibody, Tspan30 antibody, LAMP-3 antibody, MLA1 antibody, OMA81H antibody, TSPAN30 antibody, TSP29FA antibody, CD63 antigen antibody, CD63 molecule antibody, Cd63 molecule antibody, Cd63 antibody, CD63 antibody

Background: CD63, a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.Synonyms: Granulophysin, ME491, MLA1, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, TSPAN30, Tetraspanin-30

Gene ID: 967

UniProt: P08962