GLB1
Reactivity: E. coli
WB, ELISA, IP, DB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Immunocytochemistry. Western blot. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH ~7.4, 15 mM Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf Life: one year from despatch.
Expiry Date
12 months
Tahirovic, Hellal, Neukirchen, Hindges, Garvalov, Flynn, Stradal, Chrostek-Grashoff, Brakebusch, Bradke: "Rac1 regulates neuronal polarization through the WAVE complex." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 30, Issue 20, pp. 6930-43, (2010) (PubMed).
Dráber, Slavícková, Sládecek, Viklický: "Monoclonal antibodies to Escherichia coli beta-galactosidase and their use for detection and purification of recombinant expression products." in: Hybridoma, Vol. 11, Issue 3, pp. 385-90, (1992) (PubMed).
Bazil, Horejsí, Baudys, Kristofová, Strominger, Kostka, Hilgert: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, (1987) (PubMed).
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used.Synonyms: Beta-Gal Fusion Protein, Beta-Gal tag, JW0335, Lactase, b0344, lacZ tag