This antibody recognizes PECAM-1 (CD31). Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (3). SM272LE is recommended for use in Functional Studies.
No Cross-Reactivity
Human
Cross-Reactivity (Details)
Species reactivity (tested):Rat.
Purification
Affinity C Chromatography on Protein G
Immunogen
Activated, Lewis rat derived microglial cells. Spleen cells from immunised BALB/c mouse were fused with cells of the mouse SP2 myeloma cell line.
ELISA. Western Blot. Flow Cytometry: 1/10 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections: 1/10 - 1/100. Does not work on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD31, also known as platelet endothelial cell adhesion molecule 1 (PECAM1), is a type I integral membrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is constitutively expressed on the surface of endothelial cells, and concentrated at the junction between them. It is also weakly expressed on many peripheral lymphoid cells and platelets. CD31 has been used to measure angiogenesis in association with tumor recurrence. Other studies have also indicated that CD31 and CD34 can be used as markers for myeloid progenitor cells and recognize different subsets of myeloid leukemia infiltrates (granular sarcomas). CD31 is a type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule