CD43 antibody
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- Target See all CD43 (SPN) Antibodies
- CD43 (SPN) (Sialophorin (SPN))
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Reactivity
- Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD43 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity (Details)
- Species reactivity (tested):Rat.
- Purification
- Protein G Chromatography
- Immunogen
- Rat thymocyte membrane glycoproteins
- Clone
- W3-13
- Isotype
- IgG1
- Top Product
- Discover our top product SPN Primary Antibody
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- Application Notes
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Flow Cytometry (Use 10 μL of 1/50 - 1/100 diluted antibody to label 10^6 cells in 100 μL, this product is routinely tested in flow cytometry on rat splenocytes). Immunohistochemistry on frozen sections and paraffin sections (requires antigen retrieval using heat treatment prior to staining).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline pH 7.2 with 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD43 (SPN) (Sialophorin (SPN))
- Alternative Name
- CD43 / Leukosialin (SPN Products)
- Synonyms
- CD43 antibody, GALGP antibody, GPL115 antibody, LSN antibody, A630014B01Rik antibody, Cd43 antibody, Galgp antibody, Ly-48 antibody, Ly48 antibody, Lsn antibody, Lsn1 antibody, gpL115 antibody, sialophorin antibody, SPN antibody, Spn antibody
- Background
- CD43 is one of the major glycoproteins of thymocytes and T lymphocytes, it plays a role in the physicochemical properties of the T cell surface and in lectin binding. CD43 presents carbohydrate ligands to selectins. It has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. The antigen is a counter receptor for SN/Siglec1. During T cell activation CD43 is actively removed from the T cell antigen presenting cell contact site suggesting a negative regulatory role in adaptive immune response. CD43 is expressed by the majority of leucocytes, with the exception of most B cells. However CD43 expression is seen in some cases of B cell lymphocytic lymphoma and centrocytic lymphoma.Synonyms: Galactoglycoprotein, Leukocyte sialoglycoprotein, SPN, Sialophorin
- Gene ID
- 10116
- UniProt
- P13838
- Pathways
- Regulation of Leukocyte Mediated Immunity
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