This monoclonal antibody reacts with the 50 kDa surface glycoprotein LFA-2, designated as CD2. LFA-2 is the receptor for LFA-3. This antibody labels all peripheral T cells and most thymocytes but does not label B cells or peritoneal macrophages. It does not activate T cells.
Purification
Protein G Chromatography
Immunogen
T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen. Donor: BALB/c spleen Fusion Partner: NSO/1
CD2
Reactivity: Human
FACS, IP
Host: Mouse
Monoclonal
LT2
unconjugated
Application Notes
Immunohistochemistry on frozen and paraffin sections. Flow Cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS,no preservative.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD2 is a surface antigen of the human T lymphocyte lineage that is expressed on all peripheral blood T cells. It is one of the earliest T cell markers, being present on more than 95 % of thymocytes, it is also found on some natural killer cells but not on B lymphocytes. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 to mediate adhesion between T cells and other cell types. CD2 is implicated in the triggering of T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for the identification of lymphomas and leukaemias of T cell origin.Synonyms: Erythrocyte receptor, LFA-2, LFA-3 receptor, Rosette receptor, SRBC, T-cell surface antigen CD2, T-cell surface antigen T11/Leu-5