P-Selectin antibody

Catalog No. ABIN120298

Product Details anti-P-Selectin Antibody

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivity: Human, Rat, Sheep, Goat

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Selectin antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: SM2262P recognises the CD62P cell surface antigen, a 140 kDa glycoprotein also known as P-selectin. Clone Psel.KO.2.12 is reported to inhibit P-Selectin-dependent adhesion between HL60 cells and P-Selectin transfected COS cells (1). We recommend the use of SM2262LE for this purpose.

Purification: Affinity Chromatography on Protein G

Immunogen: CD62P transfected 300.19 cells. Remarks: Spleen cells from immunised CD62P knock-out mice (Strain C57/B6) were fused with cells of the NS-1 myeloma cell line.

Clone: Psel-KO-2-12

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 106 cells in 100 μL. Immunohistology on Frozen Sections. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for P-Selectin

Target: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Alternative Name: CD62P / P-Selectin (SELP Products)

Background: CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.Synonyms: CD62 antigen-like family member P, GMP-140, GMRP, GRMP, Granule membrane protein 140, LECAM3, Leukocyte-endothelial cell adhesion molecule 3, PADGEM, SELP

Gene ID: 6403

NCBI Accession: NP_002996

UniProt: P16109