hCG antibody

Catalog No. ABIN120103

Product Details anti-hCG Antibody

Target: hCG (Chorionic Gonadotropin (hCG))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This hCG antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody shows strong reaction in RIA with hCG and b-hCG with some reactivity on hLH (12 %) and b-hLH (34 %). No reaction with hFSH, hTSH, a-hCG and a-hLH.Aff. Const.:1.6 x 10e9m (Ka)

Purification: Ion exchange chromatography

Immunogen: hCG. Spleen cells from an immunised BALB/c mouse were fused with cells of the mouse myeloma cell line P3 - NS1/1 - Ag 4.1.

Clone: INN-hCG-22

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. ELISA: 1:100 - 1:500. Western Blot: Non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for hCG

Target: hCG (Chorionic Gonadotropin (hCG))

Abstract: hCG Products

Synonyms: CGB antibody, AW108023 antibody, HCG antibody, chorionic gonadotropin, beta polypeptide antibody, ubiquitin-like 3 antibody, chorionic gonadotropin beta subunit 5 antibody, CGB antibody, Ubl3 antibody, CGB5 antibody

Target Type: Hormone

Background: Human chorionic gonadotropin (hCG) is a glycoprotein hormone produced by trophoblastic cells of the placenta beginning 10 to 12 days after conception. Maintenance of the fetus in the first trimester of pregnancy requires the production of hCG, which binds to the corpus luteum of the ovary which is stimulated to produce progesterone which in turn maintains the secretory endometrium. hCG is present only in trace amounts in non pregnant urine and sera. It rises sharply during pregnancy. HCG is composed of two non identical, non covalently linked polypeptide chains designated as the a and b subunits. The a subunit of HCG is nearly identical to that of thyroid stimulating hormone (TSH), follicle stimulating hormone (FSH) and luteinizing hormone (LH). A germ cell tumor which is positive for cytokeratin, placental alkaline phosphatase (PLAP) and HCG but negative for EMA and AFP is probably a choriocarcinoma.Synonyms: CGB, CGB3, Choriogonadotropin subunit beta, beta hCG, hCG-beta

Gene ID: 1082

NCBI Accession: NP_000728

UniProt: P01233