CD32/CD16 antibody (FITC)

Catalog No. ABIN119964

Product Details anti-CD32/CD16 Antibody (FITC)

Target: CD32/CD16

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD32/CD16 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Purification: Affinity chromatography on Protein G

Immunogen: PU5 1.8 IOE7 Balb/c mouse cell line. Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.

Clone: FCR 4G8

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Neat - 1/10, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD32/CD16

Target: CD32/CD16

Alternative Name: CD16/CD32 (CD32/CD16 Products)

Background: In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.Synonyms: CD16a, FCG3, FCGR3A, Fc gamma receptor IIB, Fc-gamma RIII, Fc-gamma RIII-alpha, Fc-gamma RIIIa, Fc-gamma-RIIB, FcR mouse Seroblock, FcRII, FcRIII, FcRIIIa, Fcgr2, IGFR3, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A, Low affinity immunoglobulin gamma Fc region receptor III-B, Ly-17