Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
Specificity
This antibody is specific for Interleukin-2 (IL-2). This antibody recognises both natural and recombinant mouse IL-2. Clone JES6-1A12 is reported to function as a neutralizing antibody. We recommends the use of SM1698A for this purpose.
IL2
Reactivity: Human
WB, ELISA, FACS
Host: Rabbit
Monoclonal
unconjugated
Application Notes
ELISA: 1 μg/mL - 4 μg/mL. Immunoprecipitation. Western Blot: 1 μg/mL - 5 μg/mL. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.