TLR4 antibody (FITC)
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- Target See all TLR4 Antibodies
- TLR4 (Toll-Like Receptor 4 (TLR4))
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Reactivity
- Human, Dog, Pig, Guinea Pig, Monkey
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This TLR4 antibody is conjugated to FITC
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises the human Toll like receptor 4 (TLR4) cell surface antigen. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of SM1677LE for this purpose.
- Purification
- Affinity Chromatography on Protein G.
- Immunogen
- Ba/F3 cell line expressing TLR4 (CD284). Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
- Clone
- HTA125
- Isotype
- IgG2a
- Top Product
- Discover our top product TLR4 Primary Antibody
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anti-Toll-Like Receptor 4 (TLR4) (AA 751-835) antibody
TLR4 Reactivity: Human, Mouse, Rat, Cow WB, FACS, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibodyTLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 49-247) antibodyTLR4 Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Alkaline Phosphatase (AP))TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal Alkaline Phosphatase (AP)
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (HRP)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal HRP
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (FITC)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal FITC
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (Biotin)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal Biotin
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PE)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal PE
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (APC)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal APC
anti-Toll-Like Receptor 4 (TLR4) (AA 420-435) antibody (PerCP)TLR4 Reactivity: Human WB, FACS, IHC, IF, ICC Host: Rabbit Polyclonal PerCP
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- Application Notes
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Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 l whole blood.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- PBS, 0.09 % Sodium Azide, 1 % BSA
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- TLR4 (Toll-Like Receptor 4 (TLR4))
- Alternative Name
- CD284 / TLR4 (TLR4 Products)
- Background
- TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.Synonyms: Toll-like receptor 4
- Gene ID
- 7099, 9606
- UniProt
- O00206
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins
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