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Poly-ADP-Ribose antibody

PAR Reactivity: Human WB, IHC (p), IHC (fro), IP Host: Mouse Monoclonal 10H unconjugated
Catalog No. ABIN118982
  • Target See all Poly-ADP-Ribose (PAR) products
    Poly-ADP-Ribose (PAR)
    Reactivity
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 2
    Mouse
    Clonality
    • 2
    Monoclonal
    Conjugate
    • 2
    This Poly-ADP-Ribose antibody is un-conjugated
    Application
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Species reactivity (expected):Broad.
    Species reactivity (tested):Human.
    Purification
    Purified
    Immunogen
    Purified poly (ADP-ribose) polymer, 10-50 unit chain length.
    Clone
    10H
    Isotype
    IgG3
  • Application Notes
    Immunohistology on frozen sections: This product requires protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining. Immunoprecipitation. Western Blot: 1/500.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    PBS, 50 % Glycerol
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Poly-ADP-Ribose (PAR)
    Abstract
    PAR Products
    Synonyms
    2310036I02Rik antibody, AW260507 antibody, Emv66 antibody, par antibody, AFG3-like AAA ATPase 2 antibody, Afg3l2 antibody
    Background
    PADPR (Poly(ADP-ribose)) is a polymer synthesized by a class of enzymes named poly(ADP-ribose) polymerases (PARP). Using NAD+ as substrate, PARP catalyzes the formation of the polymer PADPR, with chain lengths ranging from 2 to 300 residues, containing approximately 2 % branching in the chain. PADPR becomes attached to nuclear proteins, and to PARP itself (automodification). Under normal conditions, cells display low basal level of PADPR polymer, which can dramatically increase in cells exposed to DNA damaging agents (irradiation, alkylation, etc.). This increase of polymer synthesis is usually transient and is followed by a rapid degradation phase with a short half life which can be less than 1 min. The low endogenous level of polymer in unstimulated cells and its rapid catabolism during DNA damage has been ascribed to high activity of the polymer catabolizing enzyme poly(ADP-ribose) glycohydrolyase (PARG).Synonyms: PADPR
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