Several Defensins are recognized. This antibody recognises a family of Cyclic Peptides in Neutrophils. Human: Defensin 1-3 (HNP-1 to HNP-3) in Human Neutrophils. Synthetic Defensin-1 and -2 (Bachem, Bubendorf CH) stain also positively. Not tested with Defensin-4. A side reaction to elastin has been observed in humans. Other: not tested. Defensins have been described in rabbits (NP-1 to NP-5, NP3a = Corticostatin), and in mice (cryptodin).
Purification
Affinity chromatography
Immunogen
Native defensins. The epitope has not been further characterized.
DEFA1
Reactivity: Human
WB, ELISA, Func
Host: Goat
Polyclonal
unconjugated
Application Notes
Immunohistochemistry on Frozen Sections: 0.2 μg/mL (1/1000). Immunohistochemistry on Paraffin Sections: 0.5 μg/mL (1/400), Proteinase K pretreatmentfor antigen retrieval is recommended. Positive Control: Human tonsil. Has been discribed to work in FACS with permeabilized cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Defensins are a family of cyclic peptides containing 29-35 amino acids (MW < 3500) which tend to form aggregates. Four of these peptides are described in humans (HNP-1 to 4), six in rabbits (NP-1 to 5). Synonyms are MCP-1 for NP-1, MCP-2 for NP-2 and corticostatin for NP-3. Biological Functions: Various functions have been described for defensins. They are antibacterial, antifungal, chemotactic for monocytes, inhibitory for ACTH-induced corticosteroid synthesis and cytotoxic for cells. Defensins are inhibited by glucosaminoglycans (self protection for cells) and high concentrations of Ca2+ ions.Synonyms: DEF-1, DEF-3, DEF-A1, DEF-A2, DEF-A3, DEF1, DEF3, DEFA-1, DEFA-2, DEFA-3, DEFA1, DEFA2, DEFA3, Defensin alpha 1, Defensin alpha 3, HNP-1, HNP-3, HNP1, HNP3, HP-1, HP-3, HP1, HP3, MRS, Neutrophil defensin 1, Neutrophil defensin 3