F-Actin antibody

Catalog No. ABIN118960

Product Details anti-F-Actin Antibody

Target: F-Actin

Reactivity: Human, Mouse, Rat, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This F-Actin antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Specificity: This antibody recognizes Human Filamentous Actin (F-actin). The antibody binds to the N-terminal region of Actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. Clone NH3 is reported to recognise Actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule. Clone NH3 is also described to show reactivity with a 43 kDa polypeptide using cell lines U937 and HL-60 by SDS/PAGE and Immunoblotting.

Purification: Purified

Immunogen: Human monocytes and U937 cell line. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Clone: NH3

Isotype: IgM

Application Details

Application Notes: Flow Cytometry (1:10). Immunohistochemistry on frozen and paraffin sections. This product may require protein digestion pretreatment (e.g. trypsin or pronase) or microwave antigen retrieval prior to staining of paraffin sections. ELISA (1:10) Western blotting (1:100 - 1:500)
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for F-Actin

Target: F-Actin

Alternative Name: Actin (F-Actin) (F-Actin Products)

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. G-Actin (globular actin) with bound ATP can polymerise to form F-Actin (Filamentious Actin). Actin monomers spiral ariound the axis of the filament similar to a double helix. F-Actin may also undergo a process called treadmilling, in which filament length remains constant and actin monomers add at one end and dissociated at the other. Synonyms: Actin F type