HLA-ABC antibody

Catalog No. ABIN118900

Product Details anti-HLA-ABC Antibody

Target: HLA-ABC

Reactivity: Human, Cow, Cat, Monkey, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-ABC antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

No Cross-Reactivity: Goat, Dog (Canine), Guinea Pig, Rabbit, Mouse (Murine), Chicken, Amphibian

Cross-Reactivity (Details): Species reactivity (tested):Human, Cynomolgus monkey, Rhesus monkey, Chimpanzee, Macaque, Bovine, Baboon, Gorilla, Shrew.

Purification: Affinity Chromatography on Protein G

Immunogen: Purified human tonsil lymphocyte membranes

Clone: W6-32

Isotype: IgG2a

Application Details

Application Notes: ELISA. Immunoprecipitation. Immunohistochemistry on frozen sections. (Does not work on paraffin sections). Flow cytometry (1:50 - 1:100): use 10 μL of diluted antibody to label 1x10^6 cells or 100 μL whole blood. This product is routinely tested in flow cytometry on human peripheral blood lymphocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-HLA-ABC antibody (ABIN118900)

Stern, Goldman, Hatasaka, Mackenzie, Surrey, Racowsky,: "Optimizing the number of cleavage stage embryos to transfer on day 3 in women 38 years of age and older: a Society for Assisted Reproductive Technology database study." in: Fertility and sterility, (2008) (PubMed).

Mayr, Kofler, Büning, Bund, Hallek, Wendtner: "Transduction of CLL cells by CD40 ligand enhances an antigen-specific immune recognition by autologous T cells." in: Blood, Vol. 106, Issue 9, pp. 3223-6, (2005) (PubMed).

Mayr, Bund, Schlee, Moosmann, Kofler, Hallek, Wendtner: "Fibromodulin as a novel tumor-associated antigen (TAA) in chronic lymphocytic leukemia (CLL), which allows expansion of specific CD8+ autologous T lymphocytes." in: Blood, Vol. 105, Issue 4, pp. 1566-73, (2005) (PubMed).

Target Details for HLA-ABC

Target: HLA-ABC

Alternative Name: HLA Class I ABC (HLA-ABC Products)

Synonyms: HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody

Background: The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class I proteins encoded by the HLA which are HLA A, HLA B and HLA C. These proteins are found on the surface of almost all nucleated somatic cells.Synonyms: HLA Class 2 ABC, Human Leukocyte antigen class I ABC, MHC class I ABC, Major Histocompatibility complex class I

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints