Recognizes the Integrin alpha 2 subunit (CD49b), which is a 160 kDa glycoprotein that non-covalently associates with the 130 kDa integrin beta1 subunit to form the VLA-2 complex.
Flow Cytometry: Use 10 μL of neat antibody to label 10^6 cells or 100 μL human whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water.
Buffer
PBS, pH 7.2 with 0.09 % Sodium Azide as preservative, 1 % BSA and 5 % Sucrose.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells. CD49b is a 160kD glycoprotein that non-covalently associates with the 130kD integrin beta 1 subunit to form the VLA-2 complex. CD49b is expressed by platelets, long term cultivated T cells, approximately 50 % of monocytes and most adherent cell lines.Synonyms: CD49 antigen-like family member B, Collagen Receptor, GPIa, ITGA-2, Integrin alpha-2, Platelet membrane glycoprotein Ia, VLA-2 alpha, VLA2