This antibody binds to the Human CD39 cell surface antigen. This antibody has been shown to block MHC independent target cell recognition by hapten-specific CTL. SM1101A is recommended for this purpose.
Purification
Affinity Chromatography on Protein G.
Immunogen
PHA activated human lymphocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells or 100 μL whole blood. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.1 mg/mL
Buffer
PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CD39 is an 80kD molecule expressed on peripheral blood B cells, monocytes and T cell clones. CD39 is also weakly expressed on granulocytes. CD39 has intrinsic ecto-ATPase activity. Expression of CD39 is induced on T cells and increased on B cells as a late activation antigen.Synonyms: ATPDase, Ecto-ATP diphosphohydrolase, Ecto-apyrase, Ectonucleoside triphosphate diphosphohydrolase 1, Lymphoid cell activation antigen, NTPDase 1