CD14 antibody
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- Target See all CD14 Antibodies
- CD14
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Reactivity
- Human, Monkey, Pig, Cow, Sheep, Dog, Goat, Cat, Rabbit, Mink
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This CD14 antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA)
- Purification
- Affinity chromatography on Protein G.
- Clone
- Tük4
- Isotype
- IgG2a
- Top Product
- Discover our top product CD14 Primary Antibody
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- Application Notes
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Flow Cytometry: 1/50-1/200, use 5 μL of suggested working dilution to label 1x10^6 cells or 100 μL whole blood. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS containing 0.09 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD14
- Alternative Name
- CD14 (CD14 Products)
- Synonyms
- CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody
- Background
- CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein
- Gene ID
- 9606
- UniProt
- P08571
- Pathways
- TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades
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