MHC Class II (RT1Bu) antibody
Reactivity: Rat, Mouse
FACS, IHC (fro), IHC (p)
Host: Mouse
Monoclonal
OX-3
unconjugated
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- Target
- MHC Class II (RT1Bu)
- Reactivity
- Rat, Mouse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purification
- Affinity chromatography
- Immunogen
- Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Clone
- OX-3
- Isotype
- IgG1
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-
- Application Notes
-
Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on Lewis rat splenocytes). Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- MHC Class II (RT1Bu)
- Alternative Name
- MHC Class II RT1Bu
- Gene ID
- 10116
- UniProt
- Q70RH8
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