Macrophage/Monocyte antibody (FITC)

Catalog No. ABIN118286

Product Details

Target: Macrophage/Monocyte

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: FITC

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody recognizes an intracellular antigen of Mouse Macrophages and Monocytes. It reacts strongly with Macrophages in lymphoid organs such as tingible body Macrophages and Macrophages in T cell dependent areas. Reacts on all Mouse strains tested.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G

Immunogen: Mouse lymph node stroma. Remarks: Spleen cells from immunised Wistar rats were fused with cells of the SP/0 myeloma cell line.

Clone: MOMA-2

Isotype: IgG2b

Application Details

Application Notes: Flow Cytometry: Use 10 μL of neat-1/10 diluted antibody to label 10^6 cells in 100 μL (membrane permeabilisation is recommended).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details

Target: Macrophage/Monocyte

Alternative Name: Macrophages / Monocytes

Background: Monocyte and macrophage are white blood cells that roam the body tissues engulfing foreign organisms. A monocyte is a leukocyte, part of the human body's immune system that protects against blood-borne pathogens and moves quickly (aprox. 8-12 hours) to sites of infection in the tissues. Monocytes are usually identified in stained smears by their large bi-lobed nucleus. Macrophages are cells within the tissues that originate from specific white blood cells called monocytes. Monocytes and macrophages are phagocytes, acting in both nonspecific defense (or innate immunity) as well as specific defense (or cell-mediated immunity) of vertebrate animals. Their role is to phagocytize (engulf and then digest) cellular debris and pathogens either as stationary or mobile cells, and to stimulate lymphocytes and other immune cells to respond to the pathogen.Synonyms: Macrophage marker, Monocyte marker