CD4 antibody
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- Target See all CD4 Antibodies
- CD4
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Reactivity
- Mouse
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Host
- Rat
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Clonality
- Monoclonal
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Conjugate
- This CD4 antibody is un-conjugated
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Application
- Flow Cytometry (FACS)
- Specificity
- This antibody recognises the murine CD4 cell surface antigen, expressed by a subset of T lymphocytes.
- Purification
- Protein G affinity chromatography
- Clone
- YTS191-1
- Isotype
- IgG2b
- Top Product
- Discover our top product CD4 Primary Antibody
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- Application Notes
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Flow cytometry: 1/50 - 1/100, use 10 μL of the suggested working dilution to label 10^6 cellsin 100 μL. Immunohistochemistry on frozen sections (3).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- PBS, pH 7.4, containing 0.09 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- CD4
- Alternative Name
- CD4 (CD4 Products)
- Background
- CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
- Gene ID
- 12504
- NCBI Accession
- NP_038516
- UniProt
- P06332
- Pathways
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
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