Biotin antibody (HRP)

Catalog No. ABIN118070

Product Details anti-Biotin Antibody (HRP)

Target: Biotin

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This Biotin antibody is conjugated to HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purification: Immunoaffinity chromatography

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)

Application Details

Application Notes: Immunoblotting (Western or dot blot), ELISA, immunoperoxidase electron microscopy andimmunohistochemistry as well as other peroxidase-antibody based enzymatic assaysrequiring lot-to-lot consistency. Recommended dilutions: Western blot 1: 500 - 1: 3,000. ELISA 1: 5,000 - 1: 25,000. Immunohistochemistry 1: 300 - 1: 1,500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water or equivalent.

Concentration: 1.0 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Gentamicin Sulfate, 10 mg/mL BSA (IgG and Protease free)

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 4 °C prior to restoration. Restore with deionized water (or equivalent), centrifuge product if not completely clear after standing at room temperature. This product is stable for four weeks at 4 °C as an undiluted liquid. For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.

Target Details for Biotin

Target: Biotin

Abstract: Biotin Products

Target Type: Chemical

Background: Biotin is a water soluble vitamin, generally classified as a B complex vitamin, also called vitamin B4. After the initial discovery of biotin, nearly forty years of research were required to establish it as a vitamin. Biotin is required by all organisms but can only be synthesized by bacteria, yeasts, molds, algae, and some plant species. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da.