Reactivity occurs against human p29 ING4 protein. 100 % homology is on record for this protein from human, dog, rat, and mouse. Cross reactivity with p29 ING4 protein from chimpanzee and chicken sources is also expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p29 ING4 proteins from other sources is not known.
Purification
Immunoaffinity chromatography.
Immunogen
A synthetic peptide corresponding aa 164-175 of Human p29 ING4 protein. This sequence only shows homology to isoform 1 for ING4. Genename: ING4
Antibody R1606P has been tested for use in ELISA (1: 3,000-1: 12,000) against theimmunizing peptide. Specific conditions for Western blotting (1: 500-1: 3,000) reactivity
Restrictions
For Research Use only
Concentration
1.28 mg/mL (by UV absorbance at 280 nm)
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
This product is photosensitive and should be protected from light
Storage
4 °C
Storage Comment
Store undiluted at 2-8 °C. DO NOT FREEZE!
Wang, He, Wang, Wang, Zhai, Zhou, Zhang, Zhai, Feng, Han: "[Impact of Donor KIR2DS5 Genotype on Outcome Following Haploidentical Hematopoietic Stem Cell Transplantation.]" in: Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology, Vol. 16, Issue 1, pp. 111-5, (2008) (PubMed).
Kim, Choi, Park, Han, Kim, Chu, Kim: "Endometrial stromal sarcoma of the small bowel: a case report and review of literature." in: Annals of diagnostic pathology, Vol. 12, Issue 2, pp. 128-33, (2008) (PubMed).
Target
ING4
(Inhibitor of Growth Family, Member 4 (ING4))
wu:fb11d09 antibody, wu:fb50e02 antibody, zgc:110619 antibody, ING4 antibody, my036 antibody, p29ING4 antibody, D6Wsu147e antibody, D6Xrf92 antibody, inhibitor of growth family, member 4 antibody, inhibitor of growth family member 4 antibody, ing4 antibody, ING4 antibody, Ing4 antibody
Background
P29 ING4 is a tumor suppressor protein similar to ING1 that may inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. p29 ING4 has been shown to suppress brain tumor angio-genesis through transcriptional repression of RelA/ NFKB3 target genes when complexed with RelA. p29 ING4 may also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. p29 ING4 shows a nuclear localization and interacts with EP300, TP53, RelA, inhibits cell growth, and induces apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. Multiple alternatively spliced transcript variants have been observed. The accession number listed below is for variant (1) that encodes the longest isoform.Synonyms: Inhibitor of growth protein 4, My036, p29ING4