CBX4 antibody (AA 95-107)

Catalog No. ABIN118002

Product Details anti-CBX4 Antibody

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Binding Specificity: AA 95-107

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human PC2 protein. Cross reactivity with PC2 protein from mouse may occur as a 92 % homology (BLAST) is noted for the immunized sequence. Cross reactivity with PC2 protein from other sources is not known.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 95-107 of Human PC2 protein.

Application Details

Application Notes: This affinity purified antibody has been tested for use in immunofluorescence staining ofcultured cells and western blotting using transfected cell lysates. The antibody is alsofunctional by ELISA. Reactivity in other immunoassays is unknown. Dilutions for westernblotting represent a starting point dilution and further optimization may be required. Theantibody detects a band of approximately 82 kDa (predicted molecular weight: 61.4 kDa). In immunofluoresence, this antibody detects the expected discrete nuclear structure thatis termed the PcG body, corresponding to the known localization of PC2. The antibody hasbeen successfully used to detect FLAG-tagged transfected hPC2 (see figure below). Itdetects a weak band that probably corresponds to endogenous PC2, however, a strongsecondary band is also seen at 50 kD in all cell lines thus far tested. This suggests that theantibody may also react with another highly expressed ubiquitous protein.

Restrictions: For Research Use only

Handling

Concentration: 0.8 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 2-8 °C.

Target Details for CBX4

Target: CBX4 (Chromobox Homolog 4 (CBX4))

Alternative Name: CBX4 / PC2 (CBX4 Products)

Background: PC2 is the human homolog of the Drosophila 'Polycomb' (Pc) protein which has been identified as a gene family member associated with a cellular memory system that is responsible for the inheritance of gene activity by progeny cells. The human Pc homolog gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins are shown to colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. The human protein is believed to function as a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation. Other reports describe PC2 as a protein that has SUMO E3 activity for the corepressors CtBP and CtBP2.Synonyms: Chromobox protein homolog 4, E3 SUMO-protein ligase CBX4, Polycomb 2 homolog

Gene ID: 8535

NCBI Accession: NP_003646

UniProt: O00257