SETDB1 antibody (AA 1058-1075)

Catalog No. ABIN117938

Product Details anti-SETDB1 Antibody

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Binding Specificity: AA 1058-1075

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SETDB1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: Human ESET, 1291 aa, predicted MW 143.2 kDa: 1 msslpgcigl daatatvese eiaelqqavv eelgismeel rhfideelek mdcvqqrkkq 61 laeletwviq kesevahvdq fddasravt nceslvkdfy sklglqyrds ssedessrpt 121 eiieipdedd dvlsidsgda srtpkdqkl reamaalrks aqdvqkfmda vnkksssqdl 181 hkgtlsqmsg elskdgdliv smrilgkkrt ktwhkgtlia iqtvgpgkky kvkfdnkgks 241 llsgnhiayd yhppadklyv gsrvvakykd gnqvwlyagi vaetpnvknk lrfliffddg 301 yasyvtqsel ypicrplkkt wediediscr dfieeyvtay pnrpmvllks gqlikteweg 361 twwksrveev dgslvrilfl ddkrcewiyr gstrlepmfs mktssasale kkqgqlrtrp 421 mgavrskgp vvqytqdltg tgtqfkpvep pqptappapp fppapplspq agdsdlesql 481 qsrkqvakk stsfrpgsvg sghssptspa lsenvsggkp ginqtyrspl gstasapaps 541 alpappappv fhgmlerapa epsyrapmek lfylphvcsy tclsrvrpmr neqyrgknpl 601 lvpllydfrr mtarrrvnrk mgfhviyktp cglclrtmqe ierylfetgc dflflemfcl 661 dpyvlvdrkf qpykpfyyil ditygkedvp lscvneidtt pppqvayske ripgkgvfin 721 tgpeflvgcd ckdgcrdksk cachqltiqa tactpggqin pnsgyqykrl eeclptgvye 781 cnkrckcdpn mctnrlvqhg lqvrlqlfkt qnkgwgircl ddiakgsfvc iyagkiltdd 841 fadkeglemg deyfanldhi esvenfkegy esdapcssds sgvdlkdqed gnsgtedpee 901 snddssddnf ckdedfstss vwrsyatrrq trgqkengls ettskdshpp dlgpphipvp 961 psipvggcnp psseetpknk vaswlscnsv seggfadsds hssfktnegg egraggsrme 1021 aekastsglg ikdegdikqa kkedtddrnk msvvtessrn ygynpspvkp eglrrppskt 1081 smhqsrrlma saqsnpddvl tlsssteseg esgtsrkpta gqtsatavds ddiqtissgs 1141 egddfedkkn mtgpmkrqva vkstrgfalk sthgiaikst nmasvdkges apvrkntrqf 1201 dgeescyii daklegnlgr ylnhscspnl fvqnvfvdth dlrfpwvaff askriragte 1261 ltwdynyevg svegkellcc cgaiecrgrl l

Specificity: This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human ESET protein. Cross reactivity with ESET protein from non-human sources is not expected based on a lack of homology (BLAST) with other known proteins.

Purification: Immunoaffinity chromatography.

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 1058-1075 of human ESET.

Application Details

Application Notes: This affinity purified antibody has been tested for use in ELISA (1: 10,000-1: 50,000) andWestern blotting (1: 1,000-1: 2,500). Western blotting shows reactivity specific for human ESET detecting a band atapproximately 170 kDa. Specific band detection by Western blot is blocked by peptidecompetition by pre-incubating the antibody with the immunizing peptide prior to reactionwith the membrane. Reactivity in other immunoassays is unknown.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.17 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SETDB1

Target: SETDB1 (SET Domain, Bifurcated 1 (SETDB1))

Alternative Name: SETDB1 (SETDB1 Products)

Synonyms: ESET antibody, H3-K9-HMTase4 antibody, KG1T antibody, KMT1E antibody, TDRD21 antibody, AU022152 antibody, mKIAA0067 antibody, SETDB1 antibody, NV10069 antibody, fc07d03 antibody, si:ch211-81a5.6 antibody, wu:fc07d03 antibody, im:7143091 antibody, wu:fa04a12 antibody, wu:fj80h02 antibody, zgc:152899 antibody, SET domain bifurcated 1 antibody, SET domain, bifurcated 1 antibody, myeloid/lymphoid or mixed-lineage leukemia; translocated to, 11 antibody, histone-lysine N-methyltransferase SETDB1 antibody, SET domain, bifurcated 1a antibody, SET domain bifurcated 1 L homeolog antibody, SET domain, bifurcated 1b antibody, SETDB1 antibody, Setdb1 antibody, MLLT11 antibody, LOC100120014 antibody, LOC100461671 antibody, setdb1a antibody, setdb1.L antibody, setdb1b antibody

Background: The SET domain is a highly conserved, approximately 150-amino acid motif implicated in the modulation of chromatin structure. It was originally identified as part of a larger conserved region present in the Drosophila trithorax protein and was subsequently identified in the Drosophila Su(var)3-9 and 'Enhancer of zeste' proteins, from which the acronym SET is derived. Studies have suggested that the SET domain may be a signature of proteins that modulate transcriptionally active or repressed chromatin states through chromatin remodeling activities. ESET functions as a histone methyltransferase by methylation of Lys-9 of histone H3. H3 Lys-9 methylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. ESET shows a nuclear localization and is associated with non-pericentromeric regions of chromatin, and is excluded from nucleoli and islands of condensed chromatin. Although ESET is a widely expressed protein, it is highly expressed in the testis.Synonyms: ERG-associated protein with SET domain, ESET, H3-K9-HMTase 4, Histone H3-K9 methyltransferase 4, Histone-lysine N-methyltransferase SETDB1, KIAA0067, KMT1E, Lysine N-methyltransferase 1E, SET domain bifurcated 1

Gene ID: 9869

NCBI Accession: NP_001138887

UniProt: Q15047