RAD23A antibody (AA 122-135)
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- Target See all RAD23A Antibodies
- RAD23A (RAD23 Homolog A (RAD23A))
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Binding Specificity
- AA 122-135
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This RAD23A antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Sequence
- Human HHR23A protein, 363 aa, predicted MW 39.6kDa: 1 mavtitlktl qqqtfkirme pdetvkvlke kieaekgrda fpvagqkliy agkilsddvp 61 irdyridekn fvvvmvtktk agqgtsappe asptaapess tsfppaptsg mshpppaare 121 dkspseesap ttspesvsgs vpssgssgre edaastlvtg seyetmltei msmgyererv 181 vaalrasynn phraveyllt gipgspepeh gsvqesqvse qpateaagen pleflrdqpq 241 fqnmrqviqq npallpallq qlgqenpqll qqisrhqeqf iqmlneppge ladisdvege 301 vgaigeeapq mnyiqvtpqe keaierlkal gfpeslviqa yfaceknenl aanfllsqnf 361 dde
- Specificity
- This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human HR23A protein. Sequence homology as assessed by BLAST indicated 100 % homology for this protein from human and rabbit. Cross reactivity with HR23A protein from dog, mouse and rat may also occur as sequence homology varies by one or two amino acid residues in this sequence by BLAST analysis. Reactivity with HR23A protein from other sources is not known.
- Purification
- Immunoaffinity chromatography.
- Immunogen
- This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 122-135 of human HR23A protein.
- Top Product
- Discover our top product RAD23A Primary Antibody
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- Application Notes
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This affinity purified antibody has been tested for use in ELISA (1: 4,000-1: 6,000) and byWestern blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 60 kDa in size corresponding to HR23A by Western blotting in theappropriate cell lysate or extract.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.2 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- RAD23A (RAD23 Homolog A (RAD23A))
- Alternative Name
- RAD23A (RAD23A Products)
- Synonyms
- rad23a antibody, wu:fe11h06 antibody, zgc:92001 antibody, RAD23A antibody, HHR23A antibody, HR23A antibody, 2310040P19Rik antibody, AL024030 antibody, mHR23A antibody, RAD23 homolog A, nucleotide excision repair protein a antibody, RAD23 homolog A, nucleotide excision repair protein antibody, rad23aa antibody, RAD23A antibody, Rad23a antibody
- Background
- HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23A, UV excision repair protein RAD23 homolog A
- Gene ID
- 5886
- NCBI Accession
- NP_005044
- UniProt
- P54725
- Pathways
- DNA Damage Repair
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