Cyanine Dyes antibody

Catalog No. ABIN117890

Product Details

Target: Cyanine Dyes

Host: Sheep

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)

Purification: Immunoaffinity Chromatography using a Cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: A mixture of Cy3 and Cy5 conjugated KLH.

Application Details

Application Notes: Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunologicalmethods requiring lot-to-lot consistency, high titer and specificity. Recommended Dilutions: ELISA: 1/10,000-1/50,000. Western blot: 1/1,000-1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.06 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride containing 0.01 % (w/v) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C prior to opening. Centrifuge product if not completely clear after standing at room temperature. For extended storage aliquot contents and freeze at -20 °C or below.

References

van den Borne, Isobe, Verjans, Petrov, Lovhaug, Li, Zandbergen, Ni, Frederik, Zhou, Arbo, Rogstad, Cuthbertson, Chettibi, Reutelingsperger, Blankesteijn, Smits, Daemen, Zannad, Vannan, Narula, Pitt et al.: "Molecular imaging of interstitial alterations in remodeling myocardium after myocardial infarction. ..." in: Journal of the American College of Cardiology, Vol. 52, Issue 24, pp. 2017-28, (2009) (PubMed).

Target Details

Target: Cyanine Dyes

Target Type: Chemical