Cyanine Dyes antibody (HRP)
IP
Host: Sheep
Polyclonal
HRP
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- Target
- Cyanine Dyes
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- Immunoprecipitation (IP)
- Purification
- Immunoaffinity chromatography
- Immunogen
- A mixture of Cy3 and Cy5 conjugated KLH
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- Application Notes
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Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase ElectronMicroscopy and Immunohistochemistry as well as other peroxidase-antibody basedenzymatic assays requiring extremely low background levels, absence of F(c) mediatedbinding, lot-to-lot consistency, high titer and specificity. Recommended Dilution(s): This product has been assayed against 1.0 g of Cyanineconjugated Sheep IgG in a standard capture ELISA using ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 1,500 to 1: 6,000 is suggested for this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % Gentamicin Sulfate as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
- Preservative
- Gentamicin sulfate
- Precaution of Use
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- Cyanine Dyes
- Target Type
- Chemical
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