Cyanine Dyes antibody (Biotin)
IF, EIA, FACS, IHC (fro)
Host: Sheep
Polyclonal
Biotin
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- Target
- Cyanine Dyes
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Host
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Sheep
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Characteristics
- Molar Ratio: 10-20 BAC molecule per sheep IgG molecule.
- Purification
- Immunoaffinity chromatography
- Immunogen
- A mixture of Cy3 and Cy5 conjugated KLH
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- Application Notes
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assay using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended dilutions: This product has been assayed against 1.0 μg of Cyanine IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 8,000 to 1: 40,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Restore with 1.0 mL of deionized water (or equivalent).
- Concentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % Sodium Azide as preservative and 10 mg/mL BSA as stabilzer, IgG and Protease free.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- Target
- Cyanine Dyes
- Target Type
- Chemical
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