TCR beta antibody

Catalog No. ABIN1177302

Product Details anti-TCR beta Antibody

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This TCR beta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: TCR affinity-purified from mouse T-cell hybridoma DO-11.10

Clone: H57-597

Isotype: IgG2 lambda

Application Details

Application Notes: Flow cytometry: It has been observed that pre-incubation of thymus cell suspensions at 37°C for 2-4 hours prior to staining enhances the ability of anti-CD3e and anti-TCR beta chain mAbs to detect the T cell receptor on immature thymocytes.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-TCR beta antibody (ABIN1177302)

Saint-Ruf, Panigada, Azogui, Debey, von Boehmer, Grassi: "Different initiation of pre-TCR and gammadeltaTCR signalling." in: Nature, Vol. 406, Issue 6795, pp. 524-7, (2000) (PubMed).

Vicari, Zlotnik: "Mouse NK1.1+ T cells: a new family of T cells." in: Immunology today, Vol. 17, Issue 2, pp. 71-6, (1996) (PubMed).

Atsuta, Nishimura, Nakamura, Emoto, Iwatsuki, Yoshikai: "Diversity of V gamma gene segments rearranged to the J gamma 4 gene in mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 2, pp. 676-84, (1995) (PubMed).

Davenport, Kumar, Bennett: "Rapid rejection of H2k and H2k/b bone marrow cell grafts by CD8+ T cells and NK cells in irradiated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 8, pp. 3742-9, (1995) (PubMed).

Bendelac, Killeen, Littman, Schwartz: "A subset of CD4+ thymocytes selected by MHC class I molecules." in: Science (New York, N.Y.), Vol. 263, Issue 5154, pp. 1774-8, (1994) (PubMed).

Ohno, Ono, Hirayama, Shimada, Saito: "Preferential usage of the Fc receptor gamma chain in the T cell antigen receptor complex by gamma/delta T cells localized in epithelia." in: The Journal of experimental medicine, Vol. 179, Issue 1, pp. 365-9, (1994) (PubMed).

Saint-Ruf, Ungewiss, Groettrup, Bruno, Fehling, von Boehmer: "Analysis and expression of a cloned pre-T cell receptor gene." in: Science (New York, N.Y.), Vol. 266, Issue 5188, pp. 1208-12, (1994) (PubMed).

Groettrup, von Boehmer: "T cell receptor beta chain dimers on immature thymocytes from normal mice." in: European journal of immunology, Vol. 23, Issue 6, pp. 1393-6, (1993) (PubMed).

Skeen, Ziegler: "Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 971-84, (1993) (PubMed).

Lefrancois: "Phenotypic complexity of intraepithelial lymphocytes of the small intestine." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 6, pp. 1746-51, (1991) (PubMed).

Bonneville, Itohara, Krecko, Mombaerts, Ishida, Katsuki, Berns, Farr, Janeway, Tonegawa: "Transgenic mice demonstrate that epithelial homing of gamma/delta T cells is determined by cell lineages independent of T cell receptor specificity." in: The Journal of experimental medicine, Vol. 171, Issue 4, pp. 1015-26, (1990) (PubMed).

Gascoigne: "Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3." in: The Journal of biological chemistry, Vol. 265, Issue 16, pp. 9296-301, (1990) (PubMed).

Kubo, Born, Kappler, Marrack, Pigeon: "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 8, pp. 2736-42, (1989) (PubMed).

Target Details for TCR beta

Target: TCR beta (T Cell Receptor (TCR) beta (TCR beta))

Abstract: TCR beta Products

Synonyms: TCRbeta antibody, Tib antibody, T cell receptor beta chain antibody, Tcrb antibody

Background: The H57-597 antibody reacts with a common epitope of the beta chain of the T-cell Receptor (TCR) complex on alphabeta TCR-expressing thymocytes and peripheral T lymphocytes and NK1.1+ thymocytes and NK-T cells of all mouse strains tested. It does not react with gammadelta TCR-bearing T cells. In the fetal and adult thymus, the TCR beta-chain may form homodimers or pair with the pre-TCR alpha-chain on the surface of immature thymocytes before expression of the TCR alpha-chain. Plate-bound or soluble H57-597 antibody activates alphabeta TCR-bearing T cells, and plate-bound mAb can induce apoptotic death.