H2-T23 antibody (AA 161-179)

Catalog No. ABIN1177269

Product Details anti-H2-T23 Antibody

Target: H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))

Binding Specificity: AA 161-179

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This H2-T23 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Qa-1[b] aa. 161-179 Peptide

Clone: 6A8-6F10-1A6

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: Since this antigen is expressed at low density on the cell surface, it may be desirable to amplify staining by using a biotinylated second-step antibody (such as anti-mouse IgG1 mAb A85-1, Cat. No. 553441) followed by a bright third-step reagent such as PE Streptavidin (Cat. No. 554061).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-H2-T23 antibody (ABIN1177269)

Vance, Jamieson, Raulet: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).

Lo, Ong, Metcalf, Soloski: "T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5398-406, (1999) (PubMed).

Salcedo, Bousso, Ljunggren, Kourilsky, Abastado: "The Qa-1b molecule binds to a large subpopulation of murine NK cells." in: European journal of immunology, Vol. 28, Issue 12, pp. 4356-61, (1999) (PubMed).

Sivakumar, Gunturi, Salcedo, Schatzle, Lai, Kurepa, Pitcher, Seaman, Lemonnier, Bennett, Forman, Kumar: "Cutting edge: expression of functional CD94/NKG2A inhibitory receptors on fetal NK1.1+Ly-49- cells: a possible mechanism of tolerance during NK cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6976-80, (1999) (PubMed).

Vance, Kraft, Altman, Jensen, Raulet: "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." in: The Journal of experimental medicine, Vol. 188, Issue 10, pp. 1841-8, (1999) (PubMed).

Noble, Zhao, Cantor: "Suppression of immune responses by CD8 cells. II. Qa-1 on activated B cells stimulates CD8 cell suppression of T helper 2 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 566-71, (1998) (PubMed).

Soloski, DeCloux, Aldrich, Forman: "Structural and functional characteristics of the class IB molecule, Qa-1." in: Immunological reviews, Vol. 147, pp. 67-89, (1996) (PubMed).

Target Details for H2-T23

Target: H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))

Alternative Name: Qa-1 b

Synonyms: 37b antibody, 37c antibody, H-2T23 antibody, H2-Qa1 antibody, Qa-1 antibody, Qa-1(b) antibody, Qa1 antibody, Qed-1 antibody, T18c antibody, T18c(37) antibody, T23b antibody, T23d antibody, histocompatibility 2, T region locus 23 antibody, H2-T23 antibody

Background: The 6A8.6F10.1A6 monoclonal antibody reacts with the Qa-1[b] alloantigen, which is a nonclassical MHC class I (Class Ib) molecule encoded by the T23 gene of the H-2 complex. Qa-1 associates with beta2-microglobulin and is expressed at low levels on most leukocytes and many other cell types. Its level of cell-surface expression is upregulated by IFNgamma or specific peptides. Qa-1 is an oligomorphic molecule which presents a limited pool of peptides to T lymphocytes bearing alphabeta and gammadeltaTCR and binds to a large subpopulation of NK cells. In fact, Qa-1[b] is the ligand for CD94/NKG2A, CD94/NKG2C, and CD94/NKG2E receptors, which are expressed on NK cells. Furthermore, it has been reported that Qa-1 expressed on activated B lymphocytes is involved in immunoregulation by inducing T-cell-mediated suppression of antibody responses. The 6A8.6F10.1A6 mAb can detect Qa-1[b] on activated splenocytes from C57BL/6 and BALB/c mice (both Qa-1[b]), but not from A/j mice (Qa-1[a]), and it can block target cell recognition by CTLs specific for Qa-1-presented antigen.

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process