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H2-T23 antibody (AA 161-179)

H2-T23 Reactivity: Mouse FACS, IP, WB, BR Host: Mouse Monoclonal 6A8-6F10-1A6 unconjugated
Catalog No. ABIN1177269
  • Target See all H2-T23 products
    H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))
    Binding Specificity
    AA 161-179
    Reactivity
    Mouse
    Host
    • 1
    Mouse
    Clonality
    • 1
    Monoclonal
    Conjugate
    • 1
    This H2-T23 antibody is un-conjugated
    Application
    Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Blocking Reagent (BR)
    Brand
    BD Pharmingen™
    Purification
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Sterility
    0.2 μm filtered
    Endotoxin Level
    Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
    Immunogen
    Qa-1[b] aa. 161-179 Peptide
    Clone
    6A8-6F10-1A6
    Isotype
    IgG1 kappa
    Top Product
    Discover our top product H2-T23 Primary Antibody
  • Application Notes
    Flow cytometry: Since this antigen is expressed at low density on the cell surface, it may be desirable to amplify staining by using a biotinylated second-step antibody (such as anti-mouse IgG1 mAb A85-1, Cat. No. 553441) followed by a bright third-step reagent such as PE Streptavidin (Cat. No. 554061).
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
    Preservative
    Azide free
    Storage
    4 °C
    Storage Comment
    Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
  • Vance, Jamieson, Raulet: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).

    Lo, Ong, Metcalf, Soloski: "T cell responses to Gram-negative intracellular bacterial pathogens: a role for CD8+ T cells in immunity to Salmonella infection and the involvement of MHC class Ib molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5398-406, (1999) (PubMed).

    Salcedo, Bousso, Ljunggren, Kourilsky, Abastado: "The Qa-1b molecule binds to a large subpopulation of murine NK cells." in: European journal of immunology, Vol. 28, Issue 12, pp. 4356-61, (1999) (PubMed).

    Sivakumar, Gunturi, Salcedo, Schatzle, Lai, Kurepa, Pitcher, Seaman, Lemonnier, Bennett, Forman, Kumar: "Cutting edge: expression of functional CD94/NKG2A inhibitory receptors on fetal NK1.1+Ly-49- cells: a possible mechanism of tolerance during NK cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 6976-80, (1999) (PubMed).

    Vance, Kraft, Altman, Jensen, Raulet: "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." in: The Journal of experimental medicine, Vol. 188, Issue 10, pp. 1841-8, (1999) (PubMed).

    Noble, Zhao, Cantor: "Suppression of immune responses by CD8 cells. II. Qa-1 on activated B cells stimulates CD8 cell suppression of T helper 2 responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 2, pp. 566-71, (1998) (PubMed).

    Soloski, DeCloux, Aldrich, Forman: "Structural and functional characteristics of the class IB molecule, Qa-1." in: Immunological reviews, Vol. 147, pp. 67-89, (1996) (PubMed).

  • Target
    H2-T23 (Histocompatibility 2, T Region Locus 23 (H2-T23))
    Alternative Name
    Qa-1 b
    Synonyms
    37b antibody, 37c antibody, H-2T23 antibody, H2-Qa1 antibody, Qa-1 antibody, Qa-1(b) antibody, Qa1 antibody, Qed-1 antibody, T18c antibody, T18c(37) antibody, T23b antibody, T23d antibody, histocompatibility 2, T region locus 23 antibody, H2-T23 antibody
    Background
    The 6A8.6F10.1A6 monoclonal antibody reacts with the Qa-1[b] alloantigen, which is a nonclassical MHC class I (Class Ib) molecule encoded by the T23 gene of the H-2 complex. Qa-1 associates with beta2-microglobulin and is expressed at low levels on most leukocytes and many other cell types. Its level of cell-surface expression is upregulated by IFNgamma or specific peptides. Qa-1 is an oligomorphic molecule which presents a limited pool of peptides to T lymphocytes bearing alphabeta and gammadeltaTCR and binds to a large subpopulation of NK cells. In fact, Qa-1[b] is the ligand for CD94/NKG2A, CD94/NKG2C, and CD94/NKG2E receptors, which are expressed on NK cells. Furthermore, it has been reported that Qa-1 expressed on activated B lymphocytes is involved in immunoregulation by inducing T-cell-mediated suppression of antibody responses. The 6A8.6F10.1A6 mAb can detect Qa-1[b] on activated splenocytes from C57BL/6 and BALB/c mice (both Qa-1[b]), but not from A/j mice (Qa-1[a]), and it can block target cell recognition by CTLs specific for Qa-1-presented antigen.
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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