HLA-ABC antibody

Catalog No. ABIN1177256

Product Details anti-HLA-ABC Antibody

Target: HLA-ABC

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-ABC antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Polyclonal Human NK Cell Line

Clone: DX17

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-HLA-ABC antibody (ABIN1177256)

Gumperz, Litwin, Phillips, Lanier, Parham: "The Bw4 public epitope of HLA-B molecules confers reactivity with natural killer cell clones that express NKB1, a putative HLA receptor." in: The Journal of experimental medicine, Vol. 181, Issue 3, pp. 1133-44, (1995) (PubMed).

Lanier, Gumperz, Parham, Melero, López-Botet, Phillips: "The NKB1 and HP-3E4 NK cells receptors are structurally distinct glycoproteins and independently recognize polymorphic HLA-B and HLA-C molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 7, pp. 3320-7, (1995) (PubMed).

Morton, Kirsch, Nance, Evans, Korman, Strominger: "Orientation of loci within the human major histocompatibility complex by chromosomal in situ hybridization." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 9, pp. 2816-20, (1984) (PubMed).

Bach, Amos: "Hu-1: Major histocompatibility locus in man." in: Science (New York, N.Y.), Vol. 156, Issue 3781, pp. 1506-8 (PubMed).

Target Details for HLA-ABC

Target: HLA-ABC

Alternative Name: HLA-A/B/C (HLA-ABC Products)

Synonyms: HLAA antibody, major histocompatibility complex, class I, A antibody, HLA-A antibody

Background: The DX17 monoclonal antibody reacts with a monomorphic epitope expressed on all HLA (human leukocyte antigen) class I molecules examined. DX17 immunoprecipitates HLA class I heavy chains (45 kDa) and beta2-microglobulin (12kDa) from radiolabeled human cell lines. HLA is determined by a complex segment of the short arm of chromosome 6 and there are many human HLAs encoded in this segment. The antigenic agglomerate is called MHC, for major histocompatibility complex. Examples of class I loci are HLA-A, -B, and -C, which are serologically assayed, class II loci, e.g., HLA-D/DR and DC1, are tested by lymphocytotoxic methods.

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints