MAP2K1
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Sample Volume
5 μL
Restrictions
For Research Use only
Format
Liquid
Buffer
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Frémin, Meloche: "From basic research to clinical development of MEK1/2 inhibitors for cancer therapy." in: Journal of hematology & oncology, Vol. 3, pp. 8, (2010) (PubMed).
Yoon, Seger: "The extracellular signal-regulated kinase: multiple substrates regulate diverse cellular functions." in: Growth factors (Chur, Switzerland), Vol. 24, Issue 1, pp. 21-44, (2006) (PubMed).
Kolch: "Meaningful relationships: the regulation of the Ras/Raf/MEK/ERK pathway by protein interactions." in: The Biochemical journal, Vol. 351 Pt 2, Issue 2, pp. 289-305, (2001) (PubMed).
Papin, Eychène, Brunet, Pagès, Pouysségur, Calothy, Barnier: "B-Raf protein isoforms interact with and phosphorylate Mek-1 on serine residues 218 and 222." in: Oncogene, Vol. 10, Issue 8, pp. 1647-51, (1995) (PubMed).
Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, (1992) (PubMed).
Target
MEK1 (MAP2K1)
(Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
The O24-836 monoclonal antibody specifically binds to the human MEK1 pS218 and MEK2 pS222 phosphorylated sites. MEK1 and MEK2 (MEK1/MEK2) are ~45 kDa dual-specificity protein kinases that are also known as MAPK/ERK Kinase 1 and 2 and MAP kinase kinases (aka, MAP2K1 and 2, MAPKK1 and 2, or MKK1 and 2). MEK1 and MEK2 activation is dependent upon phosphorylation of certain amino acid residues including serine 218 (pS218) of MEK1 and serine 222 (pS222) of MEK2 by MAP kinase kinase kinases (MAP3Ks), such as by Raf kinases. MEK1/MEK2 can phosphorylate and thus activate the MAP (Mitogen-Activated Protein) kinases, ERK1 and ERK2. The ERK kinases are critically involved in multiple signal transduction pathways induced by growth factors, cytokines, and mitogens. These kinases regulate cellular growth, differentiation, survival, motility and angiogenesis. Dysregulated activation of MEK1/2 is observed in tumor cells. During antibody development, the purified O24-836 monoclonal antibody was found to detect phosphorylated MEK1/MEK2 by Western blot analysis of cellular lysates and by indirect immunofluorescent staining and flow cytometric analysis of fixed and permeabilized cells. This antibody crossreacts with phosphorylated MEK1/MEK2 expressed by mouse cells as tested by Western blot analysis and immunofluorescent staining and flow cytometric analysis. Synonyms: MAP2K1/2, MAPKK1/2, MAP kinase kinase 1/2, MAP2K1/2, PRKMK1/2