IL3RA
Reactivity: Human
FACS, ELISA
Host: Rabbit
Monoclonal
DM31
unconjugated
Application Notes
This antibody has been found to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors and can neutralize IL-3 bioactivity. In a TF-1 cell proliferation assay, this antibody (at ~0.5 µg/ml) was found to inhibit by 50% the proliferation induced by 0.3 ng/ml (~1 U/ml) of human IL-3. In functional experiments, the 7G3 antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. In various formats, i.e., purified with azide (Cat. No. 554527), biotinylated (Cat. No. 554528), and phycoerythrin-labeled (Cat. No. 554529), the 7G3 antibody is useful for applications such as immunoprecipitation, Western blotting, and immunofluorescent labeling of cells for flow cytometric analysis.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
Preservative
Azide free
Storage
4 °C
Storage Comment
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Sun, Woodcock, Rapoport, Stomski, Korpelainen, Bagley, Goodall, Smith, Gamble, Vadas, Lopez: "Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist." in: Blood, Vol. 87, Issue 1, pp. 83-92, (1996) (PubMed).
Reacts with human CD123, the 70 kD IL-3 receptor alpha chain (IL-3Ralpha), which associates with the 120-140 kD beta subunit. The beta chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Ralpha is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors. In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. This antibody has been reported to be useful for immunoprecipitation, western blot and immunofluorescent staining for flow cytometry. At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123. This antibody is routinely tested by LAL for endotoxin level and by immunofluorescent staining and flow cytometry. Synonyms: IL-3 Receptor alpha chain