CD44 antibody

Catalog No. ABIN1176866

Product Details anti-CD44 Antibody

Target: CD44

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Blocking Reagent (BR)

Brand: BD Pharmingen™

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Sterility: 0.2 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

Immunogen: Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Clone: IM7

Isotype: IgG2b kappa

Application Details

Application Notes: Note: Investigators may wish to consider using MN 550538 for the immunohistochemistry application. In addition, anti-mouse CD44 mAb KM114 (Cat. No. 558739) has been reported to be effective for western blot analysis and blocking of hyaluronan binding.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References for anti-CD44 antibody (ABIN1176866)

Ikawa, Kawamoto, Fujimoto, Katsura: "Commitment of common T/Natural killer (NK) progenitors to unipotent T and NK progenitors in the murine fetal thymus revealed by a single progenitor assay." in: The Journal of experimental medicine, Vol. 190, Issue 11, pp. 1617-26, (2000) (PubMed).

Parish, Brennan, Cooke: "Anti-CD44 treatment does not prevent the extravasation of autopathogenic T cells to the thyroid in experimental autoimmune thyroiditis." in: Immunology, Vol. 97, Issue 3, pp. 533-9, (2000) (PubMed).

Nedvetzki, Walmsley, Alpert, Williams, Feldmann, Naor: "CD44 involvement in experimental collagen-induced arthritis (CIA)." in: Journal of autoimmunity, Vol. 13, Issue 1, pp. 39-47, (1999) (PubMed).

Brocke, Piercy, Steinman, Weissman, Veromaa: "Antibodies to CD44 and integrin alpha4, but not L-selectin, prevent central nervous system inflammation and experimental encephalomyelitis by blocking secondary leukocyte recruitment." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 12, pp. 6896-901, (1999) (PubMed).

Matsumoto, Nghiem, Nozaki, Schmits, Penninger: "Cooperation between CD44 and LFA-1/CD11a adhesion receptors in lymphokine-activated killer cell cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 12, pp. 5781-9, (1998) (PubMed).

Naor, Sionov, Ish-Shalom: "CD44: structure, function, and association with the malignant process." in: Advances in cancer research, Vol. 71, Issue 4, pp. 241-319, (1997) (PubMed).

Weiss, Sleeman, Renkl, Dittmar, Termeer, Taxis, Howells, Hofmann, Köhler, Schöpf, Ponta, Herrlich, Simon: "An essential role for CD44 variant isoforms in epidermal Langerhans cell and blood dendritic cell function." in: The Journal of cell biology, Vol. 137, Issue 5, pp. 1137-47, (1997) (PubMed).

Weber, Ashkar, Glimcher, Cantor: "Receptor-ligand interaction between CD44 and osteopontin (Eta-1)." in: Science (New York, N.Y.), Vol. 271, Issue 5248, pp. 509-12, (1996) (PubMed).

Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, (1995) (PubMed).

Katoh, Zheng, Oritani, Shimozato, Kincade: "Glycosylation of CD44 negatively regulates its recognition of hyaluronan." in: The Journal of experimental medicine, Vol. 182, Issue 2, pp. 419-29, (1995) (PubMed).

Hathcock, Hirano, Murakami, Hodes: "CD44 expression on activated B cells. Differential capacity for CD44-dependent binding to hyaluronic acid." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 12, pp. 6712-22, (1994) (PubMed).

Katoh, McCarthy, Kincade: "Characterization of soluble CD44 in the circulation of mice. Levels are affected by immune activity and tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 8, pp. 3440-9, (1994) (PubMed).

Naujokas, Morin, Anderson, Peterson, Miller: "The chondroitin sulfate form of invariant chain can enhance stimulation of T cell responses through interaction with CD44." in: Cell, Vol. 74, Issue 2, pp. 257-68, (1993) (PubMed).

Camp, Scheynius, Johansson, Puré: "CD44 is necessary for optimal contact allergic responses but is not required for normal leukocyte extravasation." in: The Journal of experimental medicine, Vol. 178, Issue 2, pp. 497-507, (1993) (PubMed).

Lesley, Hyman, Kincade: "CD44 and its interaction with extracellular matrix." in: Advances in immunology, Vol. 54, Issue 4, pp. 271-335, (1993) (PubMed).

Vremec, Zorbas, Scollay, Saunders, Ardavin, Wu, Shortman: "The surface phenotype of dendritic cells purified from mouse thymus and spleen: investigation of the CD8 expression by a subpopulation of dendritic cells." in: The Journal of experimental medicine, Vol. 176, Issue 1, pp. 47-58, (1992) (PubMed).

MacDonald, Budd, Cerottini: "Pgp-1 (Ly 24) as a marker of murine memory T lymphocytes." in: Current topics in microbiology and immunology, Vol. 159, Issue 4, pp. 97-109, (1990) (PubMed).

Spangrude, Klein, Heimfeld, Aihara, Weissman: "Two monoclonal antibodies identify thymic-repopulating cells in mouse bone marrow." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 142, Issue 2, pp. 425-30, (1989) (PubMed).

Lynch, Ceredig: "Mouse strain variation in Ly-24 (Pgp-1) expression by peripheral T cells and thymocytes: implications for T cell differentiation." in: European journal of immunology, Vol. 19, Issue 2, pp. 223-9, (1989) (PubMed).

Budd, Cerottini, Horvath, Bron, Pedrazzini, Howe, MacDonald: "Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 10, pp. 3120-9, (1987) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44 (Pgp-1, H-CAM, Ly24) (CD44 Products)

Synonyms: CDW44 antibody, CSPG8 antibody, ECMR-III antibody, HCELL antibody, HUTCH-I antibody, IN antibody, LHR antibody, MC56 antibody, MDU2 antibody, MDU3 antibody, MIC4 antibody, Pgp1 antibody, AU023126 antibody, AW121933 antibody, AW146109 antibody, HERMES antibody, Ly-24 antibody, Pgp-1 antibody, CD44A antibody, METAA antibody, RHAMM antibody, XCD44 antibody, cd44 antibody, cdw44 antibody, cspg8 antibody, ecmr-iii antibody, hcell antibody, lhr antibody, mc56 antibody, mdu2 antibody, mdu3 antibody, mic4 antibody, mutch-i antibody, pgp1 antibody, CD44 molecule (Indian blood group) antibody, syndecan 4 antibody, CD44 antigen antibody, CD44 molecule (Indian blood group) S homeolog antibody, CD44 antibody, SDC4 antibody, Cd44 antibody, cd44.S antibody

Background: The IM7 antibody reacts with an epitope on both alloantigens and all isoforms of the CD44 glycoprotein (Pgp-1, Ly-24). The standard form of CD44, lacking variable exons and referred to as CD44H or CD44s, is widely expressed on hematopoietic and non-hematopoietic cells. CD44 isoforms encoded by variable exons are expressed on epithelial cells, but only at low levels on most leukocytes. Mice with the Ly-24.1 alloantigen (e.g., BALB/c, CBA/J, DBA/1, DBA/2) have relatively large subsets of CD44H+ T lymphocytes, while Ly-24.2 strains (e.g., A, AKR, CBA/N, C3H/He, C57BL, C57BR, C57L, C58, NZB, SJL, SWR, 129) have few CD44H+ T cells. CD44 is a cell adhesion receptor, and its principal ligand, hyaluronate, is a common component of extracellular matrices. Differential glycosylation of CD44 influences its binding to hyaluronate. Additional ligands include the cell-surface form of CD74 and the cytokine osteopontin (Eta-1). Bone marrow- and thymus-derived progenitor cells capable of repopulating the thymus express CD44. In the periphery, the level of CD44 expression increases upon activation of B lymphocytes, CD4+ T cells, and CD8+ T cells, memory cells can be recognized by their CD44[hi] phenotype. The IM7 mAb inhibits established collagen-induced arthritis in DBA/1 mice. Moreover, it prevents CNS inflammation and clinical symptoms of experimental autoimmune encephalomyelitis. In contrast, the same antibody exacerbates experimental autoimmune thyroiditis in CBA/J mice. The IM7 mAb recognizes a different epitope from that recognized by mAb KM114 (Cat. No. 558739), and the antibody pair can be used in ELISA to detect soluble CD44. It has been observed that IM7 antibody cross-reacts with human, dog, cat, horse, cow, and pig leukocytes. Anti-human CD44, clone G44-26 (Cat. No. 555476), and IM7 antibody compete for binding to human peripheral blood lymphocytes.
Synonyms: Pgp-1, H-CAM, Ly-24

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling