HMGB1 antibody
(1 reference)-
- Target See all HMGB1 Antibodies
- HMGB1 (High Mobility Group Box 1 (HMGB1))
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Reactivity
- Mammalian
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HMGB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking Antibody (Inhibition), Cellular Assay (CA)
- Immunogen
- 17-mer peptide KGKPDAAKKGVVKAEKS
- Clone
- DPH1-1
- Isotype
- IgG1
- Top Product
- Discover our top product HMGB1 Primary Antibody
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anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibodyHMGB1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 2F6 unconjugated
anti-High Mobility Group Box 1 (HMGB1) antibodyKO Validated HMGB1 Reactivity: Human WB, IHC, IF, ICC, IHC (p), IHC (wm), IHC (ff) Host: Rabbit Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibodyHMGB1 Reactivity: Human WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 Reactivity: Mouse WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 Reactivity: Rat WB, IHC, ICC, IP Host: Rabbit Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1D5 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 Reactivity: Human WB, ELISA, IF, IHC (p) Host: Mouse Monoclonal 1E6-E10 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 124-154), (C-Term) antibodyHMGB1 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal 1# unconjugated
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- Application Notes
- WB: 1 µg/ml antibody in 5% milk TBS-T overnight at 4°C. Migration assay: 0.5/1/2/5 µg/ml of DPH1.1 mAb is added to 30 ng/ml of rHMGB1, according to Palumbo et al. “Extracellular HMGB1, a signal of tissue damage, induces mesoangioblast migration and proliferation”.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Handling Advice
- DPH1.1 mAb is provided freeze-dried.
- Storage
- -20 °C
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: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).
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: "Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release." in: Aging, Vol. 11, Issue 22, pp. 10252-10265, (2020) (PubMed).
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- Target
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- Alternative Name
- High-mobility group protein B1 (HMGB1) (HMGB1 Products)
- Background
- DPH1.1 mAb recognizes all mammalian HMGB1s, including human, mouse and rat. Does not recognize HMGB2. DPH1.1 mAb can be used for Western blot (WB), immunofluorescence (IHF) and immunohistochemistry (IHC). DPH1.1 mAb blocks HMGB1-elicited cell migration in trans-well migration assays. In vivo, DPH1.1 mAb administered intravenously (220 µg/mouse) blocks recruitment of inflammatory cells to sites of necrosis and infection. The mouse monoclonal IgG1 DPH1.1 was generated by injecting C57BL/6 mice with the 17-mer peptide KGKPDAAKKGVVKAEKS. Hybridomas were produced from splenocytes by standard techniques and tested by ELISA against the immunogen and full-length HMGB1.
- Pathways
- p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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