CAPN1 antibody (AA 75-343)
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- Target See all CAPN1 Antibodies
- CAPN1 (Calpain 1 (CAPN1))
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Binding Specificity
- AA 75-343
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CAPN1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Calpain 1 (CAPN1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against CAPN1. It has been selected for its ability to recognize CAPN1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Calpain 1 (CAPN1) corresdonding to Pro75~Arg343 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product CAPN1 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- CAPN1 (Calpain 1 (CAPN1))
- Alternative Name
- Calpain 1 (CAPN1 Products)
- Synonyms
- CAPN1 antibody, Capa-1 antibody, Capa1 antibody, mu-calpin antibody, Capn1 antibody, fj67g01 antibody, wu:fj67g01 antibody, calpain 1 antibody, zgc:55262 antibody, CAPN1 antibody, Capn1 antibody, zgc:55262 antibody
- Background
- PIG30, CANPL1, MuCANP, MuCL, Calpain 1, Large Subunit, Calpain-1 Catalytic Subunit, Calcium-Dependent Protease 1, Calcium-activated neutral proteinase 1, Calpain mu-type, Micromolar-calpain
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