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EGFR2 antibody (AA 376-578) (FITC)

EGFR2 Reactivity: Human IHC, WB, ICC, IF Host: Rabbit Polyclonal FITC
Catalog No. ABIN1175126
  • Target See all EGFR2 Antibodies
    EGFR2 (Epidermal Growth Factor Receptor 2 (EGFR2))
    Binding Specificity
    AA 376-578
    Reactivity
    • 4
    • 3
    Human
    Host
    • 6
    • 1
    Rabbit
    Clonality
    • 6
    • 1
    Polyclonal
    Conjugate
    • 4
    • 2
    • 1
    This EGFR2 antibody is conjugated to FITC
    Application
    • 7
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Sequence
    MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS- FLPES FDGDPASNTA PLQPEQLQVF ETLEEITGYL YISAWPDSLP DLSVFQNLQV IRGRILHNGA YSLTLQGLGI SWLGLRSLRE LGSGLALIHH NTHLCFVHTV PWDQLFRNPH QALLHTANRP EDECVGEGLA CHQLCARGHC WGPGPTQCVN CSQFLRGQEC VEECRVLQGL PREYVNARHC LPCHPECQPQ NGSVTCFG
    Specificity
    It has been selected for its ability to recognize EGFR2 in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    Recombinant EGFR2 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against EGFR2 conjugated to fitc.
    Isotype
    IgG
    Top Product
    Discover our top product EGFR2 Primary Antibody
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Comment

    Content: The quality control contains recombinant EGFR2 (Phe376~Gly578) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    EGFR2 (Epidermal Growth Factor Receptor 2 (EGFR2))
    Abstract
    EGFR2 Products
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