IL5RA antibody (AA 42-182)
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- Target See all IL5RA Antibodies
- IL5RA (Interleukin 5 Receptor, alpha (IL5RA))
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Binding Specificity
- AA 42-182
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This IL5RA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against IL5Ra. It has been selected for its ability to recognize IL5Ra in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Interleukin 5 Receptor Alpha (IL5Ra) corresdonding to Gly42~Cys182 (Accession # Q01344)
- Isotype
- IgG
- Top Product
- Discover our top product IL5RA Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- IL5RA (Interleukin 5 Receptor, alpha (IL5RA))
- Alternative Name
- Interleukin 5 Receptor Alpha (IL5RA Products)
- Synonyms
- MGC86517 antibody, IL5RA antibody, il5r antibody, cdw125 antibody, hsil5r3 antibody, gpIL-5ra antibody, CD125 antibody, CDw125 antibody, HSIL5R3 antibody, IL5R antibody, Il5r antibody, interleukin 5 receptor subunit alpha L homeolog antibody, interleukin 5 receptor subunit alpha antibody, interleukin 5 receptor, alpha antibody, il5ra.L antibody, IL5RA antibody, il5ra antibody, Il5ra antibody
- Background
- CDw125, CD125, IL5R-A, IL5-RA, HSIL5R3, IL5R, IL5R-A
- Pathways
- JAK-STAT Signaling
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