EGF antibody (AA 367-489)
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- Target See all EGF Antibodies
- EGF (Epidermal Growth Factor (EGF))
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Binding Specificity
- AA 367-489
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Reactivity
- Dog
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This EGF antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Epidermal Growth Factor (EGF)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against EGF. It has been selected for its ability to recognize EGF in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Human
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Epidermal Growth Factor (EGF) corresdonding to His367~Phe489 (Accession # Q9BEA0)
- Isotype
- IgG
- Top Product
- Discover our top product EGF Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:320-1300 Immunohistochemistry: 5-20 μg/mL
1:32-130 Immunocytochemistry: 5-20 μg/mL
1:32-130 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- EGF (Epidermal Growth Factor (EGF))
- Alternative Name
- Epidermal Growth Factor (EGF Products)
- Synonyms
- HOMG4 antibody, URG antibody, AI790464 antibody, CEGF antibody, epidermal growth factor antibody, pro-epidermal growth factor antibody, EGF antibody, egf antibody, CpipJ_CPIJ020278 antibody, Egf antibody
- Background
- URG, Beta-Urogastrone, Pro-epidermal growth factor
- Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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