Cytokeratin 1 antibody (AA 487-644)
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- Target See all Cytokeratin 1 (KRT1) Antibodies
- Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))
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Binding Specificity
- AA 487-644
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cytokeratin 1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Cytokeratin 1 (CK1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against CK1. It has been selected for its ability to recognize CK1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Pig, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Cytokeratin 1 (CK1) corresdonding to Glu487~Arg644 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product KRT1 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))
- Alternative Name
- Cytokeratin 1 (KRT1 Products)
- Synonyms
- CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, Krt-2.1 antibody, Krt2-1 antibody, Krt86 antibody, KRT1 antibody, Kb1 antibody, KER1 antibody, CK-1 antibody, Keratin-1 antibody, keratin 1 antibody, KRT1 antibody, Krt1 antibody
- Background
- KRT1, EHK1, K1, KRT1A, KRT1-A, Keratin 1, Epidermolytic Hyperkeratosis, Keratin, type II cytoskeletal 1, 67 kDa cytokeratin, Type-II keratin Kb1
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