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Ubiquitin antibody (AA 1-76)

Ubiquitin Reactivity: Human WB, ELISA, IHC (p), ICC, IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1172489
  • Target See all Ubiquitin Antibodies
    Ubiquitin
    Binding Specificity
    • 18
    • 8
    • 8
    • 7
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-76
    Reactivity
    • 141
    • 70
    • 69
    • 66
    • 17
    • 17
    • 15
    • 14
    • 13
    • 13
    • 13
    • 13
    • 12
    • 11
    • 10
    • 9
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 100
    • 73
    • 3
    • 1
    • 1
    • 1
    Rabbit
    Clonality
    • 94
    • 85
    Polyclonal
    Conjugate
    • 77
    • 13
    • 12
    • 10
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Ubiquitin antibody is un-conjugated
    Application
    • 162
    • 90
    • 82
    • 60
    • 57
    • 42
    • 27
    • 21
    • 20
    • 14
    • 13
    • 9
    • 4
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Sequence
    MGHHHHHHSG SEF-MQIFVKTLTG KTITLEVEPS DTIENVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGG
    Specificity
    It has been selected for its ability to recognize Ub in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    Recombinant Ubiquitin expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against Ub.
    Isotype
    IgG
    Top Product
    Discover our top product Ubiquitin Primary Antibody
  • Comment

    Content: The quality control contains recombinant Ubiquitin (Met1~Gly76) disposed in loading buffer.
    Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
    Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
    Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    Ubiquitin
    Alternative Name
    Ubiquitin (Ub) (Ubiquitin Products)
    Pathways
    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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