Complement C4c antibody (cleaved) (Biotin)

Catalog No. ABIN1171857

Product Details anti-Complement C4c Antibody (Biotin)

Target: Complement C4c (C4c) (Complement Component C4c (C4c))

Binding Specificity: cleaved

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Complement C4c antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Specificity: It has been selected for its ability to recognize C4c in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Native Protein C4cC4b was cleaved by C3b inactivator (C3bINA) in the presence of C4b-binding protein and C4d and C4c isolated. The radioactive label and therefore the reactive thiol group were located to C4d. C4c was reduced and alkylated and the two alpha'-chain fragments of C4c were separated. The molecular weights, amino acid analyses and carbohydrate content of the three alpha'-chain fragments were determined. C4d has a mol.wt. of 44500 and a carbohydrate content of 6 % . The two alpha'-chain fragments of C4c have mol.wts. of 25000 and 12000 and carbohydrate contents of 10 and 22 % respectively.
The antibody is a rabbit polyclonal antibody raised against C4c conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for Complement C4c

Target: Complement C4c (C4c) (Complement Component C4c (C4c))

Alternative Name: Complement Component 4c (C4c) (C4c Products)