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Complement C4c antibody (cleaved) (Biotin)

C4c Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1171857
  • Target See all Complement C4c (C4c) products
    Complement C4c (C4c) (Complement Component C4c (C4c))
    Binding Specificity
    • 2
    • 1
    cleaved
    Reactivity
    Human
    Host
    • 11
    • 4
    • 2
    Rabbit
    Clonality
    • 13
    • 4
    Polyclonal
    Conjugate
    • 11
    • 2
    • 1
    • 1
    • 1
    • 1
    This Complement C4c antibody is conjugated to Biotin
    Application
    • 10
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA
    Specificity
    It has been selected for its ability to recognize C4c in immunohistochemical staining and Western blotting.
    Purification
    Affinity Chromatography
    Immunogen
    Native Protein C4cC4b was cleaved by C3b inactivator (C3bINA) in the presence of C4b-binding protein and C4d and C4c isolated. The radioactive label and therefore the reactive thiol group were located to C4d. C4c was reduced and alkylated and the two alpha'-chain fragments of C4c were separated. The molecular weights, amino acid analyses and carbohydrate content of the three alpha'-chain fragments were determined. C4d has a mol.wt. of 44500 and a carbohydrate content of 6 % . The two alpha'-chain fragments of C4c have mol.wts. of 25000 and 12000 and carbohydrate contents of 10 and 22 % respectively.
    The antibody is a rabbit polyclonal antibody raised against C4c conjugated to biotin.
    Isotype
    IgG
  • Application Notes
    Western blotting: 1:100-400
    Immunocytochemistry in formalin fixed cells: 1:100-500
    Immunohistochemistry in formalin fixed frozen section: 1:100-500
    Immunohistochemistry in paraffin section: 1:50-200
    Enzyme-linked Immunosorbent Assay: 1:100-200
    Optimal working dilutions must be determined by end user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Avoid repeated freeze/thaw cycles
    Storage
    4 °C
    Storage Comment
    Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
    Expiry Date
    12 months
  • Target
    Complement C4c (C4c) (Complement Component C4c (C4c))
    Alternative Name
    Complement Component 4c (C4c) (C4c Products)
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