Neutrophils antibody
Reactivity: Mouse
FACS, IHC, Func, ICC
Host: Rat
Monoclonal
Nimp-R14
unconjugated
(8 references)-
- Target See all Neutrophils products
- Neutrophils
- Reactivity
- Mouse
-
Host
- Rat
-
Clonality
- Monoclonal
-
Conjugate
- This Neutrophils antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (IHC), Functional Studies (Func), Immunocytochemistry (ICC)
- Specificity
- Recognizes mouse neutrophils (surface protein mouse Ly-6G) and may recognize Ly-6Chigh cells (inflammatory monocytes).
- Cross-Reactivity
- Mouse (Murine)
- Purification
- Purified from concentrated hybridoma tissue culture supernatant.
- Purity
- >95 % (SDS-PAGE)
- Endotoxin Level
- <0.1EU/μg purified protein (LAL test, Lonza).
- Immunogen
- Purified mouse BALB/c neutrophils.
- Clone
- Nimp-R14
- Isotype
- IgG2b
-
anti-Neutrophils antibody (PE)
Reactivity: Mouse FACS Host: Rat Monoclonal 07-04-14 PE
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Negative control: AG-35B-0011 http://www.adipogen.com/ag-35b-0011/rat-igg2b-isotype-control.html
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- In PBS containing 10 % glycerol and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - Expiry Date
- 12 months
-
-
: "Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity." in: International journal of oncology, Vol. 53, Issue 4, pp. 1580-1590, (2018) (PubMed).
: "Rapid recruitment of inflammatory monocytes is independent of neutrophil migration." in: Blood, Vol. 102, Issue 1, pp. 328-35, (2003) (PubMed).
: "Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 7, pp. 3883-9, (2003) (PubMed).
: "An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 5, pp. 2628-36, (2000) (PubMed).
: "A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5." in: International immunology, Vol. 12, Issue 6, pp. 899-908, (2000) (PubMed).
: "Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 8, pp. 4546-56, (1999) (PubMed).
: "Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritis in mice." in: Journal of leukocyte biology, Vol. 61, Issue 3, pp. 267-78, (1997) (PubMed).
: "Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies." in: British journal of haematology, Vol. 57, Issue 3, pp. 489-94, (1984) (PubMed).
-
: "Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity." in: International journal of oncology, Vol. 53, Issue 4, pp. 1580-1590, (2018) (PubMed).
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- Target
- Neutrophils
- Abstract
- Neutrophils Products
- Background
- Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.
- UniProt
- P35461
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