DYKDDDDK Tag antibody

Catalog No. ABIN116940

Product Details anti-DYKDDDDK Tag Antibody

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DYKDDDDK Tag antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))

Sequence: DYKDDDDK

Purification: Affinity purification of this polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with the DYKDDDDK epitope tag peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling.

Application Details

Application Notes: This antibody is optimally suited for monitoring the expression of DYKDDDDK-taggedfusion proteins. As such, this antibody can be used to identify fusion proteins containingthe DYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the aminoorcarboxy termini of targeted proteins. This antibody has been tested by ELISA andwestern blotting against both the immunizing peptide and DYKDDDDK containingrecombinant proteins. Although not tested, this antibody is likely functional for

Restrictions: For Research Use only

Handling

Concentration: 1.04 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: DYKDDDDK Epitope Tag (DYKDDDDK Tag Products)

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK.