Caspase 1 p20 antibody

Catalog No. ABIN1169380

Product Details anti-Caspase 1 p20 Antibody

Target: Caspase 1 p20

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 1 p20 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recognizes endogenous full-length and activated (p20 fragment) human caspase-1.

Cross-Reactivity: Human

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant human caspase-1.

Clone: Bally-1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 10 % glycerol and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-Caspase 1 p20 antibody (ABIN1169380)

Rolfes, Ribeiro, Hawwari, Böttcher, Rosero, Maasewerd, Santos, Próchnicki, Silva, Wanderley, Rothe, Schmidt, Stunden, Bertheloot, Rivas, Fontes, Carvalho, Cunha, Latz, Arditi, Franklin: "Platelets Fuel the Inflammasome Activation of Innate Immune Cells." in: Cell reports, Vol. 31, Issue 6, pp. 107615, (2020) (PubMed).

Hamarsheh, Osswald, Saller, Unger, De Feo, Vinnakota, Konantz, Uhl, Becker, Lübbert, Shoumariyeh, Schürch, Andrieux, Venhoff, Schmitt-Graeff, Duquesne, Pfeifer, Cooper, Lengerke, Boerries, Duyster et al.: "Oncogenic KrasG12D causes myeloproliferation via NLRP3 inflammasome activation. ..." in: Nature communications, Vol. 11, Issue 1, pp. 1659, (2020) (PubMed).

Xue, Xi, Liu, Guo, Zhang, Zhang, Li, Yang, Zhou, Yang, Zhang, Zhang, Gu, Yang, Da, Yao, Duo, Zhang: "miR-21 promotes NLRP3 inflammasome activation to mediate pyroptosis and endotoxic shock." in: Cell death & disease, Vol. 10, Issue 6, pp. 461, (2020) (PubMed).

Wu, Lu, Zhang, Zhang, Shi, Hisada, Grover, Zhang, Li, Xiang, Shi, Li, Daugherty, Smyth, Kirchhofer, Shiroishi, Shao, Mackman, Wei, Li: "Inflammasome Activation Triggers Blood Clotting and Host Death through Pyroptosis." in: Immunity, Vol. 50, Issue 6, pp. 1401-1411.e4, (2019) (PubMed).

Mehto, Jena, Nath, Chauhan, Kolapalli, Das, Sahoo, Jain, Taylor, Chauhan: "The Crohn's Disease Risk Factor IRGM Limits NLRP3 Inflammasome Activation by Impeding Its Assembly and by Mediating Its Selective Autophagy." in: Molecular cell, Vol. 73, Issue 3, pp. 429-445.e7, (2019) (PubMed).

Honda, Sadatomi, Yamamura, Nakashioya, Tanimura, Takeda: "WP1066 suppresses macrophage cell death induced by inflammasome agonists independently of its inhibitory effect on STAT3." in: Cancer science, Vol. 108, Issue 3, pp. 520-527, (2017) (PubMed).

Gaidt, Ebert, Chauhan, Ramshorn, Pinci, Zuber, ODuill, Schmid-Burgk, Hoss, Buhmann, Wittmann, Latz, Subklewe, Hornung: "The DNA Inflammasome in Human Myeloid Cells Is Initiated by a STING-Cell Death Program Upstream of NLRP3." in: Cell, Vol. 171, Issue 5, pp. 1110-1124.e18, (2017) (PubMed).

Derangère, Chevriaux, Courtaut, Bruchard, Berger, Chalmin, Causse, Limagne, Végran, Ladoire, Simon, Boireau, Hichami, Apetoh, Mignot, Ghiringhelli, Rébé: "Liver X receptor ? activation induces pyroptosis of human and murine colon cancer cells." in: Cell death and differentiation, Vol. 21, Issue 12, pp. 1914-24, (2014) (PubMed).

Target Details for Caspase 1 p20

Target: Caspase 1 p20

Alternative Name: Caspase-1 p20 (Caspase 1 p20 Products)

Background: Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1beta (IL-1beta) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

UniProt: P29466