TNFRSF10A
Reactivity: Human, Mouse, Rat
IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
In PBS containing 10 % glycerol and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Short Term Storage: +4°C Long Term Storage: -20°C Stable for at least 1 year after receipt when stored at -20°C.
Expiry Date
12 months
Secchiero, Melloni, Heikinheimo, Mannisto, Di Pietro, Iacone, Zauli: "TRAIL regulates normal erythroid maturation through an ERK-dependent pathway." in: Blood, Vol. 103, Issue 2, pp. 517-22, (2004) (PubMed).
Tecchio, Huber, Scapini, Calzetti, Margotto, Todeschini, Pilla, Martinelli, Pizzolo, Rivoltini, Cassatella: "IFNalpha-stimulated neutrophils and monocytes release a soluble form of TNF-related apoptosis-inducing ligand (TRAIL/Apo-2 ligand) displaying apoptotic activity on leukemic cells." in: Blood, Vol. 103, Issue 10, pp. 3837-44, (2004) (PubMed).
Ganten, Haas, Sykora, Stahl, Sprick, Fas, Krueger, Weigand, Grosse-Wilde, Stremmel, Krammer, Walczak: "Enhanced caspase-8 recruitment to and activation at the DISC is critical for sensitisation of human hepatocellular carcinoma cells to TRAIL-induced apoptosis by chemotherapeutic drugs." in: Cell death and differentiation, Vol. 11 Suppl 1, pp. S86-96, (2004) (PubMed).
Leverkus, Sprick, Wachter, Mengling, Baumann, Serfling, Bröcker, Goebeler, Neumann, Walczak: "Proteasome inhibition results in TRAIL sensitization of primary keratinocytes by removing the resistance-mediating block of effector caspase maturation." in: Molecular and cellular biology, Vol. 23, Issue 3, pp. 777-90, (2003) (PubMed).
Washburn, Weigand, Grosse-Wilde, Janke, Stahl, Rieser, Sprick, Schirrmacher, Walczak: "TNF-related apoptosis-inducing ligand mediates tumoricidal activity of human monocytes stimulated by Newcastle disease virus." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 4, pp. 1814-21, (2003) (PubMed).
Sprick, Rieser, Stahl, Grosse-Wilde, Weigand, Walczak: "Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8." in: The EMBO journal, Vol. 21, Issue 17, pp. 4520-30, (2002) (PubMed).
Target
TNFRSF10A
(Tumor Necrosis Factor Receptor Superfamily, Member 10a (TNFRSF10A))
TRAIL-R1 is a receptor for the cytotoxic ligand TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappaB.