Beta-Phosphoglucomutase antibody

Catalog No. ABIN116917

Product Details anti-Beta-Phosphoglucomutase Antibody

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Reactivity: Lactococcus lacti

Host: Goat

Clonality: Polyclonal

Conjugate: This Beta-Phosphoglucomutase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoprecipitation (IP)

Specificity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified beta-Phosphoglucomutase [Lactococcus lacti]. Cross reactivity against beta-Phosphoglucomutase from other sources is unknown.

Purification: Multistep process.

Immunogen: beta-Phosphoglucomutase [Lactococcus lacti].

Application Details

Application Notes: Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 mg ofbeta-Phosphoglucomutase [Lactococcus lacti] in a standard sandwich ELISA usingPeroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 3,000 to 1: 15,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Restore with 2.0 mL of deionized water (or equivalent).

Concentration: 90.0 mg/mL (by Refractometry)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

Target Details for Beta-Phosphoglucomutase

Target: Beta-Phosphoglucomutase (Phosphoglucomutase, beta-)

Abstract: Beta-Phosphoglucomutase Products

Synonyms: ECK1312 antibody, JW1310 antibody, beta-phosphoglucomutase antibody, ycjU antibody, pgmB antibody, LMOf2365_2822 antibody, Exig_2478 antibody, Hore_00430 antibody, Tmath_0800 antibody, Aazo_0214 antibody, Spirs_3639 antibody, Closa_0065 antibody, Toce_1769 antibody, Tthe_1054 antibody, Lbys_0589 antibody, Celal_0979 antibody, Sgly_0722 antibody, Celly_2426 antibody, Spico_1537 antibody, Theth_1551 antibody

Background: Synonyms: Beta-PGM, Beta-phosphoglucomutase, Glucose Phosphomutase, Phosphoglucomutase beta

Gene ID: 1114041

NCBI Accession: NP_266585

UniProt: P71447