HCV 1b NS5B antibody

Catalog No. ABIN1169140

Product Details anti-HCV 1b NS5B Antibody

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Reactivity: Hepatitis C Virus (HCV)

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCV 1b NS5B antibody is un-conjugated

Application: Immunoprecipitation (IP), Immunocytochemistry (ICC), Functional Studies (Func)

Specificity: Recognizes HCV NS5B from HCV genotype 1 (epitope mapped to NS5B aa 139-392).

Cross-Reactivity: Hepatitis C Virus (HCV)

Cross-Reactivity (Details): Does not react with genotype 2.

Purification: Purified from concentrated hybridoma tissue culture supernatant.

Purity: >95 % (SDS-PAGE)

Immunogen: Recombinant hepatitis C virus (HCV) NS5B RNA-dependent RNA polymerase.

Clone: 5B-12B7

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: In PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C.

Expiry Date: 12 months

References for anti-HCV 1b NS5B antibody (ABIN1169140)

Dimitrova, Imbert, Kieny, Schuster: "Protein-protein interactions between hepatitis C virus nonstructural proteins." in: Journal of virology, Vol. 77, Issue 9, pp. 5401-14, (2003) (PubMed).

Kapadia, Brideau-Andersen, Chisari: "Interference of hepatitis C virus RNA replication by short interfering RNAs." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 4, pp. 2014-8, (2003) (PubMed).

Moradpour, Bieck, Hügle, Wels, Wu, Hong, Blum, Bartenschlager: "Functional properties of a monoclonal antibody inhibiting the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 593-601, (2002) (PubMed).

Schmidt-Mende, Bieck, Hugle, Penin, Rice, Blum, Moradpour: "Determinants for membrane association of the hepatitis C virus RNA-dependent RNA polymerase." in: The Journal of biological chemistry, Vol. 276, Issue 47, pp. 44052-63, (2001) (PubMed).

Target Details for HCV 1b NS5B

Target: HCV 1b NS5B (HCV NS5b) (Hepatitis C Virus NS5b (HCV NS5b))

Abstract: HCV NS5b Products

Target Type: Viral Protein

Background: The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.